NEHA TAMRAKAR alien LAB REPORT BISC-260-002 05/10/11 IDENTIFYING UNKNOWN MICROORGANISM INTRODUCTION: In this lab exercise all(prenominal) single students were given an alien sample of microorganism which was numbered. The organism that I needed to constitute was number 11. All assorted manners were followed to identify the microorganism adjacent the lab manual. The gradually methods were performed which divided the organism in multitude and subgroups which at the remnant gave a specific microorganism. First method divided bacteria in 2 groups gram lordly and gram negative. accordingly the general anatomy was identified as celestial pole or Cocci. The process of identification was followed by the oxygen emergency canvass and motility test. The methods followed while identifying the unknown microorganism ar explained below. g-force STAINING: Microorganisms are not visible by bare-assed eyes. Gram staining is an empirical m ethod of differentiating bacterial species into two large groups (gram positive and gram negative) based on the chemical, in general the presence of high levels of peptidoglycan, and physical properties of their cell wall. It uses crystallizing vio endure to stain cell wall, iodine as acerb and safranin to counter strain to mark all bacteria. We utilise this to reason the microorganism between two major groups and expose the shape of the organism.
Material and method: - Crystal majestic - Grams iodine - Gloves - Slides - coils - Safranin - Ethyl alcohol - Bacteria - Burner - Slides - Loop ! Firstly, for gram stain the water and bacteria are administer over the parachute with a loop and let it straining dry. Then the slide is heat fixed over the fervidness over Bunsen burner. The slide is rinsed with crystal violet for 30 sec and washed off with water for 2 sec. the slide is then washed with grams iodine which works as bleak for a minute and the slide is washed once to a greater extent to wash the excess. Then to decolorize the excess the...If you want to chasten a full essay, order it on our website: OrderCustomPaper.com
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