Saturday, October 26, 2013

DNA Experiments

1.Experiments by the following scientists provided critical in governance concerning deoxyribonucleic acid. Fully suck out 2 of these 3 classical experiments and indicate how to each one provided conclusion for the chemical nature of the gene. a. Hershey and Chase- bacteriophage replicationThey prepared firebird rive take ins of viruses, one in which the DNA (contains phosphorus alone no sulfur) was labeled with a hot isotope, 32P, and the separate in which the protein (contains virtually sulfur and no phosphorus) was labeled with a radioactive isotope 35S. E. coli were large on a medium that contained one of the radioactive isotopes. iodin colony of bacteria was infected with P-labeled virus, and the other - with S-labeled virus. later on transmitting had begun and the viruses had transferred their genetic material into the bacteria, the mixture was spun in a blender. Thus, cardinal layers were formed: one containing extracellular material and the other - intracell ular materialThe results were the following:-the extracellular material contained 35S. This meant that protein is not inserted in bacterial cells when viruses reproduce-the intracellular material (the infected bacterial cells) contained 32P, which meant that DNA is the common carrier of genetic material that is needed for the formation of a viral progeny. b. Griffith and Avery- bacterial transformationStreptococcus pneumoniae, a bacterium that feats pneumonia, has two forms: virulent (with capsule) and non-virulent (without capsule).
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Griffith was look for for a vaccine for pneumonia and conducted a serial of expe riments. One of them (marked in bold) produc! ed a puzzling result. 1) shiner + break down encapsulated -> exsanguine2) mouse + heat-killed encapsulated -> a brook3) mouse + live nonencapsulated -> alive4) mouse + live nonencapsulated + heat-killed encapsulated -> deadA sample from a dead mouse from experiment n.4 revealed that there were live encapsulated bacteria in spite of appearance the mouse. Griffith concluded that a so-called transformation factor could cause a variation in... If you want to get a adept essay, order it on our website: OrderCustomPaper.com

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